The rapid growth of protein-based biomedical research has resulted in a need to measure protein data in ways that are precise, repeatable, and efficient. However, current immunoassays like ELISA are often the opposite – time consuming and tedious with multiple steps that can introduce variation. By harnessing the power of microscale analysis and lab automation, Correlia is accelerating improvements in the efficiency and accuracy of immunoassays. We caught up with their CSO, Samuel Tia, to find out more about Correlia's approach.
PIXI platform automates sensitive protein assays
To meet the demand for accurate, high throughput protein quantification methods, Correlia Biosystems built the PIXI module to integrate with automated liquid handling systems like the Opentrons OT-2. The PIXI module incorporates unique features to address many of the limitations inherent in traditional ELISAs and automates sample handling to reduce variability. Each assay consumable contains a set of nanoporous capture gels which reduces the time requirement for analyte capture from hours down to minutes. The PIXI immunoassay platform generates replicate data points from less than 1 μL of sample and delivers analyte quantitation with up to 5-log dynamic range (Figure 1). This preserves precious samples for downstream analyses and eliminates the need for performing multiple dilutions to get the data you need.
Automated aspiration improves assay efficiency
In working with researchers, Correlia Biosystems identified the need for improved aspirating capacity on automated liquid handling platforms for applications like ELISA and adherent cell-based assays. Their new aspirator arm (Figure 2) provides efficient liquid aspiration within the OT-2 platform. An integrated high-flow vacuum pump enables precise and rapid removal of liquid from microplate wells or tubes. The innovative solutions presented by Correlia Biosystems are made possible by the highly adaptable OT-2 platform. The modularity and open-source nature of Opentrons liquid handling robots enable the expansion and automation of unique workflows. Watch the webinar to learn more about how Correlia Biosystems is using Opentrons lab automation to streamline protein quantification in immunoassays.