An OT-One will hold up to two pipettes. A single channel pipette can be used on the left side (axis B), or the right side (axis A), but a multi channel will only fit on axis A.
If you consider all the different things you can do with a pipette by hand ... the list in your head probably isn’t that long. But there are so many variables to consider when programming a pipette, and the OT-One gives you the ability to control all of them. Here we'll give a run-down of the various elements and variables you can manipulate by using the OT-One.
Left side: filled-out parameters. Right side: default parameters.
Simply: what you want to name your pipette. Like the deck containers, this name will be used throughout the protocol, so make it something easy to type!
This will always be “pipette”. Long-term OpenTrons goals include being able to use a variety of lab equipment, but for now we’re just focusing on pipettes.
This is where you assign a tip rack to each pipette. The tip rack must exist in the deck to be called here, and the names of each must match.
As the trash container must be named “trash”, this attribute will always be the same.
A pipette is either a multi channel (True), or single channel (False).
This is where you tell the robot which pipette is on the right (a), and which is on the left (b).
The volume is the maximum volume of the pipette: 10 for a 10 uL pipette, 200 for a 200 uL, and so on. Even if you use less than the maximum volume in your experiment, this needs to be set to the maximum.
How fast the plunger is depressed is defaulted to 300, but can be adjusted up or down.
How fast the plunger is released is defaulted to 500, but, like the down-plunger-speed, it can be adjusted.
This distance is how far the pipette drops when it is instructed to pick up a tip, and should be set to 6.
If you want the robot to pull up more liquid than is set in each transfer, you can specify that extra volume here.
Once a volume has been specified for extra-pull-volume, you can tell the robot to delay for a specific amount of time before pulling up the extra volume. Like the “delay” attribute, this is in seconds. If the extra-pull-volume is 0, there will not be a delay.
For use with the distribute command, extra volume will be drawn up at the start of the command.